Microwave‐assisted extraction combined with GC‐MS for determination of volatile small molecule to evaluate compatibility of antimicrobial peptide PL‐5 spray with packaging materials

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January
08
/ 2020

Various experimental parameters were systematically optimized and good linearities were obtained within the range of 0.05‐1.00 μg/ml for DBB and DBHBA and 0.50‐10.00 μg/ml for DBP, with limits of detection (LOD) of 0.99 ng/ml (DBB), 16.34 ng/ml (DBP) and 1.64 ng/ml (DBHBA), respectively. Satisfactory recoveries that ranged from 96.0 % to 107.2 % were acquired and the relative standard deviation (RSD) was no more than 4.7 %. The results showed that the maximum daily usage of DBB, DBP and DBHBA were 9.859 ng, 163.445 ng and 16.399 ng, respectively, which were far below the corresponding PDE values according to the safety assessment, indicating that the migration of analytes did not bring any potential safety risk. The compatibility between the drug and the packaging materials was favorable.

Source url
https://onlinelibrary.wiley.com/doi/abs/10.1002/bmc.4748
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Microwave‐assisted extraction combined with GC‐MS for determination of volatile small molecule to evaluate compatibility of antimicrobial peptide PL‐5 spray with packaging materials
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Miscellaneous
Short Description
Pstronga Simple And Efficient Method Involving Microwave Assisted Extraction Mae Combined With Gcms Was Established To Determine 13ditertbutylbenzene Dbb 24ditertbutylphenol Dbp 35ditertbutyl4hydroxybenzaldehyde Dbhba For The Purpose Of Evaluating The Compatibility With Drug Packaging Materials In This Study The Antimicrobial Peptide Pl5 Spray Was Purified By A Welchrom C18 Column And The High Density Polyethylene Spray Bottle With Liquid Collection Tube Was Firstly Mixed With Absolute Ethanol Which Would Be Subjected To Mae For Further Measurement By Gcmsstrongp